Purpose: To evaluate the effect of three natural materials (Ginger and honey mixture and rosemary oil) on remineralization and prevention of enamel white spot lesions measured by color change using vita easy shade. Material and methods: The study was carried out in two phases remineralization and prevention phase, sixty four anterior teeth were used for each phase. Teeth were prepared and randomized into four groups according to treatment material (n=8 samples). Group I: control group which receives no treatment. Group II: treated with 1.23% sodium fluoride gel. Group III: treated with ginger-honey mixture. Group IV: treated with rosemary oil. Treatment material was applied for 3 or 6 minutes. In phase (1) color was measured at baseline, after creation of white spot lesion, and after treatment. In phase (2) color was measured at baseline and after treatment. Data were obtained and the differences between groups were estimated by ANOVA test followed by Tukey's post hoc test. Results: for phase1: There was a statistically significant difference among different groups. The highest mean value of ΔE was for sodium fluoride gel group followed by ginger and honey group at 6 min. For phase2: group III had a better acid resistance effect followed by group II at 6 min. Conclusion: Natural materials can be used for remineralization and prevention of WSL as ginger and honey mixture enhance remineralization following sodium fluoride gel which was more effective. And for acid resistance phase, ginger and honey mixture was more effective.