Purpose: This study was conducted to evaluate the effect of different staining solutions on color stability and surface roughness (SR) of Nano hybrid composite and acrylic denture teeth. Materials and Methods: Fifty specimens divided into two main groups. Each group divided into 5 subgroups each of 5 specimens of acrylic and composite teeth were used in this test. Teeth were immersed in four coloring solutions in addition to distilled water. Color measurements were  erformed using spectrophotometer. Color change values were determined before and after immersion in the coloring solutions. The CIE L*a*b* color space was used to evaluate color differences. SR values were evaluated before and following the immersion in coloring solutions using a digital microscope. Results: Showed significant color changes of both types of artificial teeth materials upon immersion in tea, coffee, cola, licorice or distilled water. This color change was greater in case of tea and coffee solutions. The least color change values were obtained from specimens immersed in distilled water. SR values were increased for acrylic teeth compared with composite teeth and there were significant statistical differences in surface roughness of all tested materials after immersion in any of the tested solutions. Conclusions: The acrylic denture teeth material is much more subjected to discoloration than the composite denture teeth material. Surface roughness of all tested teeth of both types recorded statistically significant decrease by immersion in staining solutions.