Purpose: In this study we were aiming to determine and evaluate the efficacy of the stem cells and corticotomy on the rate of canine retraction. Materials and Methods: 22 patients from both genders were selected. The age range of the selected cases was (15-20 years). All patients were examined to fulfill the inclusion criteria. These patients were divided into two groups; control group and intervention group. Split mouth technique was adopted in the intervention group to minimize the variables. In the intervention group one side received piezocorticomy only, while the other side received piezocorticotomy and stem cells. In control group conventional orthodontic canine retraction was carried out. To measure the rate of canine retraction; impression and casts were taken every one month till complete canine retraction. Casts were then digitally scanned and superimposed to digitally measure the rate of canine retraction. Results: Regarding maxilla the results showed no significant difference in the total rate of canine retraction of all comparative groups. Regarding mandible, the difference was significant higher between both stem cell and piezo groups in comparison to the control group. The canine retraction rate was higher in maxilla and mandible with stem cell injection than in the Piezo group of both arches; this difference in maxilla was in the first month of retraction only, but in mandible it was in the first two months. Conclusions: addition of stem cells to piezocorticotomy showed highest canine retraction rate in early months of retraction.