Purpose: Evaluation of the effect of diode laser and iontophoresis with aqueous extract of Nigella sativa and fluoride-based-agent on dentin permeability.
Materials and Methods: Thirty-six lower premolars were used to prepare 72 samples and were randomly divided into three intervention groups 24 per group; Group A1 Samples were treated with aqueous extract of Nigella sativa, Group A2 Samples were treated with fluoride-based-agent, Group A3 Control group were left without treatment. The main groups were subdivided into two subgroups of 12 samples, according to surface treatments: Group B1 was treated with diode laser and Group B2 was treated with iontophoresis. Results: Permeability results revealed that there was statistically significant difference between the study groups with the lowest mean depth of dye penetration values was found in group samples treated with aqueous extract of Nigella sativa with iontophoresis. Conclusion: Aqueous extract of Nigella sativa, a fluoride-based-agent with the application of diode laser and iontophoresis on each was effective in reducing dentin permeability, being an aqueous extract of Nigella sativa the most effective when using with iontophoresis.