Purpose: This study was designed to evaluate the autogenous bone grafting (ABG) alone or in combination with intra-marrow penetration (IMP) in the treatment of one osseous wall defects in dog model .Material and methods: This current study was conduct on 8 Beagle dogs that were alienated randomly into two groups: Group I: received open flap debridement with autogenous bone graft, Group П: received open flap debridement with autogenous bone graft and IMP. Each animal received two treatment modalities, one for each jaw. Each jaw divided into two halves (right and left). The surgery intervention was performed at base line in the left side. The right side received the same treatment after 6 weeks. All experimental defects were followed up through histological analysis. Results: Histomorphometric analysis showed significantly increased bone neogenesis within the experimental groups at 14, 60 days. The total area percent of newly formed bone in group I was 38.46+7.71% while it was 33.17+7.3% in group II with non-significant difference between two groups throughout the study intervals. Conclusion: The regenerative therapy using either autogenous bone graft alone or in combination with IMP showed an improvement in the treatment outcome of one osseous wall defects.