Purpose: As the goal of modern dentistry is the non-invasive management of non-cavitated caries lesions and the growing interest in the field of phytotherapeutics by the medicinal use of their plant extracts, this study was conducted to evaluate the remineralizing potential of some herbals (tea tree, grape seed, and ginger) in comparison with fluoridated toothpaste on initial enamel carious lesions. Materials and Methods: Forty human enamel samples were classified into four groups. Ten samples for each group were used in this in vitro recycling study with the following treatments which applied three times a day: 1) tea tree oil, 2) grape seed oil, 3) ginger oil, and 4) sodium fluoride toothpaste. Treatment regimens of demineralization and remineralization cycle were applied for 21 days. The baseline and post-treatment data were assessed by digital radiographs of the mineral density of WSLs based on subtraction concept. Data were statistically analyzed by ANOVA test with Bonferroni significant difference test. Results: There was no statistically significant difference between different study groups (fluoride, tea tree, grape seed, and ginger) along consequent follow up periods. While, after repeated measure analysis, there was a statistically significant difference regarding all the study groups respectively (0.12 (0.00), 0.22 (0.01), 0.25 (0.02), 0.12 (0.00). The post hoc test for each study group showed also a significant decrease in mineral density along different follow-up periods (P≤0.05). Conclusion: There was no statistically significant difference between different study groups on remineralization of initial caries lesion.