Purpose: This study aimed to evaluate the retention of single implant mandibular overdenture using different single crown coping materials. Materials and Methods: Clear acrylic resin mandibular cast was fabricated with single implant located in the mid-line of the cast. The cast with the implant was scanned for designing of nine copings of different materials using computer aided design computer aided manufacture CAD/CAM and according to material used for copings fabrication, models were categorized as follow: Poly etherether ketone (PEEK) group, Zirconium dioxide (ZrO2) group, hybrid composite resin coping group. Nine identical mandibular complete overdentures were constructed, tensile force for removing overdenture with copings was then recorded. Results: Comparison of recorded tensile strength results showed a significant difference among groups where the highest retention was recorded with Zirconia group, followed by PEEK and the lowest retention results were noted with Hybrid composite resin. Conclusion: Within the limitation of this in vitro study, it could be concluded that single crown that fabricated from ZrO2 for supporting implant supported overdenture showed better retention results in comparison to PEEK a hybrid composite one.