Purpose: This study was designed to investigate the remineralizing potential of some natural materials (Honey, Whey extract, Zamzam water) versus synthetic product (fluoride tooth paste) on demineralized enamel. Materials and methods: A total of 40 premolars were extracted from 11-14 years old patients. Teeth were washed with distilled water to remove debris; and stored in distilled water contain 0.1% mg thymol. Forty samples were sectioned into two parts (buccal and lingual) then divided into four main groups (20 each) according to materials used; A1: Ginger & honey, A2:Whey extract, A3:Zamzam water & ginger and A4:Fluoride tooth paste. The four groups were further subdivided into two subgroups according to the time of assessment; B1: after 20 days and B2: after 40 days. All samples were treated by demineralizing solution Coca-Cola for 8 minutes, then the samples were mounted and tested materials were applied. Samples were examined with polarized light microscopy. Results: After 20 days, the results revealed that the highest mean value of microhardness was recorded for ginger& zamzam water (273.39), followed by whey extract (256.63), followed by Ginger & honey groups (249.20) while Fluoride group showed the lowest mean of microhardness (219.56). This difference was statistically significant. After 40 days, the results revealed that the highest mean value of microhardness was recorded for Ginger & Zamzam water (266.89), followed by Ginger & honey (260.39), followed by Whey extract group (251.16) while Fluoride group showed the lowest mean of microhardness (226.44). This difference was statistically significant. Conclusion: Natural materials have remineralizing effect on enamel of tooth.