Purpose: The aim of this study was to assess root surface after platelet rich plasma (PRP) injection as a means for tooth movement acceleration. Materials and Methods: 10 female patients with age ranged between (14-19) years old were selected for this study. Total of 40 canine sites were divided into two groups; each group included 20 canines: (group I): 10 upper canines& 10 lower canines (right side – study side), (group II): 10 upper canine & 10 lower canines (left side – control side). All patients were examined to fulfill the inclusion criteria. Patients in each group underwent radiographic evaluation for the root surface after canine retraction (with & without PRP injection). The primary radiographic variables were the canine retraction rate and the root length after retraction. Results: Study duration for patients in both groups was six months. The difference was statistically significant between the two groups regarding the treatment durations, where it was 3.25 ± 0.45 and 5.63 ± 0.73 months in PRP group and conventional orthodontic therapies respectively. Actually, no significant changes occurred in clinical probing depth between both groups at the start of treatment and till the end of the 6th month. Group I resulted in decrease in root length by 0.09 mm ± 0.4, while Group II showed decrease in root length by 1.4mm ± 0.8 which is not statistically significant difference. Conclusion: PRP injection is safe easy method of retraction acceleration of the canine without affecting root length.