Purpose: The purpose of this study was to access post-operative clinical as well as radioghraphic success of Diode Laser / Mineral Trioxide Aggregate (MTA) and Diode Laser /Biodentine (BD) pulpotomy procedures in comparison to conventional technique using Formocresol /Zinc Oxide Eugenol (FC/ZOE) pulpotomy for human primary molars. Materials and Methods: A total of 90 lower primary molars that had deep carious lesion and need pulpotomy treatment from 66 healthy and cooperative patients, aged 4-6 years old were chosen for this study. After removal of the coronal tissue and depending on the type of radicular pulp medicament, the teeth were divided randomly and equally into three groups; Group A (FC/ZOE), Group B (Diode Laser/MTA) and Group C (Diode laser/BD). Treatments in all groups were completed with Glass Ionommer restoration then stainless steel crowns. Results: The cumulative clinical and radiographic success rates after 12 months follow up were 90.3%, 96.3% and 100% for the FC/ZOE, Diode Laser/MTA and Diode Laser/BD pulpotomy groups respectively. Conclusion: Laser assessed pulpotomy in combination with MTA or Biodentine had a more advanced clinical and radiographic success rate correlated to FC/ZOE pulpotomy technique in primary molar. Statistically, no significance difference was found between the three study groups in the overall clinical and radiographic success rate.