Purpose: the current study was conducted for evaluation of solubility and sealing ability of two recently introduced biocearmic root end filling materials (Bio MTA and Total Fill) in comparison to the gold standard Mineral Trioxide Aggregate (MTA).Materials and Methods: Regarding solubility test a total of 15 disc specimens were fabricated from the three tested materials. The discs were then divided randomly into three equal groups (n=5) according to tested root end filling materials (Pro root MTA, Bio MTA, and Total Fill biocearmic). The solubility test was done according to ISO no 6786. The amount of residue weight from each specimen was estimated and expressed as a percent of solubility. While for sealing ability testing, a total number of 15 freshly extracted human mandibular premolars with straight roots, single canal, and fully formed root apices were selected, and the crown was removed and canal was prepared and obturated, then the apical 3 mm of each root was removed. The root-end cavity with 3mm in depth and 1.5mm in width was done using diamond ultrasonic tips. The tested materials were mixed and placed inside the cavities. The specimens were sectioned by isomet for further environmental scanning electron microscopic investigation of sealing ability. Results: In both tests a significant difference was found between the control group (MTA group) and two tested groups (Total Fill group, Bio MTA group) where (p < 0.001).Moreover, the Bio MTA group and Total Fill group showed a lower solubility % and higher sealing ability in comparison to Pro root MTA group and this was explained in term of negative correlation between the solubility %and sealing ability. Conclusions: Total Fill and Bio MTA exhibits less solubility % and better sealing ability compared to the Pro root MTA. Based on improved sealing ability of both Total Fill and Bio MTA material, they could be considered as a good choice for the replacement of conventional MTA as root end filling materials