Purpose: This study aimed to compare the effect of two anticollagenolytic agents, chlorohexidine and Aloe vera on dentin microhardness and their effect as pretreatments on the remineralization potential of CPP-ACFP. Materials and Methods: For this study, 40 extracted sound human premolars were subjected to artificial erosion and randomly divided to two equal groups (20 premolars each) according to immersion in the tested anticollagenolytic agents (A); group (A1) Aloe vera and group (A2) Chlorhexidine. Each group was further subdivided into two equal subgroups (10 premolars each) according to microhardness measurement (B); group (B1): before CPP-ACFP application and group (B2): after CPP-ACFP application. Results: Results showed no statistically significant difference between Aloe-Vera and CHX; both statistically increased dentin microhardness. After immersion, AFCP showed statistically significantly higher mean microhardness than no AFCP application (P-value = 0.003) for Aloe vera and (P-value = 0.012) for CHX. Conclusion: Aloe vera and CPP-ACFP produced synergistic effect for enhancing dentin remineralization in erosive lesions.