Purpose: The present study was conducted to evaluate the effect of autogenous bone graft on the osseointegration of early loaded dental implant either alone or combined with Melatonin gel. Material and Methods: The study was including 10 patients with 20 numbers of sites and age range of 22-52 years. The study groups were designed in two groups, test group treated surgically with dental implant after application of autogenous bone graft mixed with (1.2mg) melatonin gel. Control group treated surgically with dental implant after application of autogenous bone graft alone. Results: Clinically, in both groups, there was change in gingival thickness with significant difference within test group. Regarding radiographic marginal bone levels measurements; in both groups the mean marginal bone loss was increased by time, with significant difference between both groups in favor of test group. Conclusions: Early loaded dental implants can be successfully used to reduce the healing period. The adjunctive topical use of melatonin seems to have a more valuable outcome in promoting the early osseointegration which reflect the meaningful in clinical and radiographic improvement.