Purpose: This study was assessed the efficacy of Apple vinegar as final irrigating solution in removing smear layer using XP-Endo Finisher File by scanning microscope. Material and Methods: Fifty single rooted lower premolars were selected and prepared using Universal ProTaper rotary files. Samples were divided into two main groups (I,II) regarding the final rinse used (20 samples each) and control group (10 samples): Group I: rinsed with 17% EDTA, Group II: rinsed with Apple vinegar and Control group: irrigated with sterile saline. Each group was subdivided into two subgroups (A,B) regarding the agitation file. Subgroup A: Conventional irrigation, Subgroup B: agitated with XP Endo Finisher. Samples were grooved longitudinally and smear layer was assessed. Results: no statistically significant difference in the median scores of smear layer produced by 17% EDTA and Apple Vinegar at coronal, middle and apical root levels when conventional irrigation or XP Endo Finisher were used. There was a significant difference between two irrigating techniques at apical level when 17% EDTA used as final rinse, where XP Endo Finisher showed statistically significant lower median score (P≤ 0.05). Conclusions: Apple vinegar presented similar smear layer removal efficiency from intraradicular dentin compared to 17% EDTA when used as final rinse. XP-Endo Finisher is a successful agitation system for removal of smear layer from intraradicular dentin.