This study was designed to assess the effect of 17% ethylenediaminetetraacetic acid (17%EDTA) and 0.2% Chitosan as chelating agents on bond strength of CPoint obturation system to root canal dentin. Materials and Methods: Fifty extracted lower premolars were selected and prepared using Universal ProTaper rotary files then the samples were equally distributed into 2 main groups depending on the type of the final rinse. Group I:17 % EDTA, Group II: 0.2% chitosan, Control group: distilled water. Each main group was further equally distributed into 2 subgroups depending on the type of the filling material. Subgroup A: samples were obturated with gutta-percha and AH Plus sealer. Subgroup B: samples were obturated with CPoint and EndoSeqence BC sealer. The push out test was carried on using universal testing machine. The sealer penetration inside the dentinal tubules was evaluated using scanning electron microscope (SEM). Results: regarding the results of subgroup A and B, at all levels, the highest value of bond strength was recorded in samples treated with 17% EDTA, while the lowest value was recorded with the control group and there was no statistical significant difference among the tested groups except for subgroup B at the middle level, there was a statistical significant difference among the tested groups. SEM observation of sealer/ dentin interface revealed few traces of sealer penetration with samples treated with 17% EDTA. Conclusion: 17% EDTA when used as final rinse produced higher bond strength with CPoint/EndoSequence BC sealer compared to 0.2% chitosan, especially at the middle third