 Purpose: The aim of this study was to evaluate the anticipated effect of both Zamzam water and Epidermal growth factor (EGF) separately on tongue tissues and associated lingual salivary glands of diabetic albino rats,( Histological and Ultrastructure evaluation) Materials and Methods Eighteen male albino rats were selected and divided into four groups. Group I (control group) received no drugs, while rats of group II (diabetic group) received a single dose of alloxan intraperitoneal injection. Moreover, rats of group III diabetic and received zamzam water for 3 months and group IV diabetic and received EGF. All rats were sacrificed; tongue was dissected and processed for hematoxylin &eosin and transmission electron microscopy evalution. Results The diabetic group showed histological & ultrastructural changes in the tongue epithelium and underlying lamina propria, a condition which almost restored to normal in zamzam treated group and relatively restored in EGF group. Concolusion Zamzam water provides safer alternative nutritional strategy in restoring the integrity of the damaged epithelium of the tongue tissues after degenerative effect of DM. EGF cause relatively improvement of the damaged epithelium especially in basal cell layer, keratin layer and blood vessels in lamina propria of tongue tissue.