Purpose: This study was designed to evaluate the chelating potential of chitosan citrate and chitosan acetate on intraradicular dentin using (ESEM) and quantity calcium content of intraradicular dentin using (EDX). Materials and Methods: sixty freshly extracted human single rooted lower premolars with single canal were instrumented with Revo-S files till size AS 35. The samples were divided into 5 experimental groups according to the final rinse used and control group (10 samples each); Group I: irrigated with 0.2% chitosan acetate, Group II: irrigated with 0.2% chitosan citrate, Group III: irrigated with 1% acetic acid, Group IV: irrigated with 1% citric acid, Group V: irrigated with 17% EDTA and control group which was divided into;(VIa) :5 samples irrigated with sterile saline and (VIb): 5 samples were neither prepared nor received any treatment. The cleanliness of the root canals and smear layer were evaluated at three root levels using ESEM. The calcium content of five samples from each experimental group (I,II,III,IV,V) and the samples of control group was measured using EDX. Results: At the coronal, middle and apical root levels; there was a statistical significant difference among the tested groups and the control . Regarding calcium content, the highest mean calcium content was recorded in samples treated with saline followed by samples that did not receive any irrigation Conclusion: The chelating potential of 0.2% chitosan acetate and 0.2% chitosan citrate is comparable to that of 17% EDTA when used as final rinse without much altering the calcium content.