Aim: This study was carried out to evaluate the effect of various chelating solutions (EDTA-Apple Vinegar-Sodium Hypochlorite)on the radicular push-out bond strength of zinc oxide nanosealer and AH Plus resin based rootcanal sealer. Materials and Methods: 180 extracted single-rooted human teeth with complete roots, fully formed apices were used in this study,on completion of instrumentation, the specimens will be randomly divided into three groups (n=60) according to chelating solution applied: Group ( I ) 1.5 mL of 17% EDTA (pH = 7), Group ( II ) 2.5 mL of vinegar apple and Group ( III ) 2 mL of 5.25% sodium hypochlorite (NaOCl). These groups are subdivided into two subgroups (n=30) each according to root canal sealer used: Subgroup A: AH Plus and Subgroup B: Zinc oxide nanosealer. Then the specimens in the subgroups will be further randomly divided into two groups (n=15) according to storage time: Subgroup 1:one week storage time and Subgroup 2: one month storage time The root canals were prepared using ProTaper rotary instruments up to master apical rotary size F3 (#30), in conjunction with 2 mL of Normal saline solution (Sodium Chloride 0.9%) irrigation between each file and the chelating solution was applied for 1 minute then final rinse with distilled water. The sealers were loaded and introduced to the canal using K file size40.Testing push-out bond strength was done for all samples using the Intrson universal testing machine. Results: It was found that the highest push-out bond strength mean value recorded for (EDTA+ AH Plus) (1 week) group (IA1) (3.424 MPa) followed by (Apple Vinegar+ AH Plus) (1 week) group (IIA1) (1.263 MPa) while the lowest mean value recorded for (NaOCl+ZnO nanosealer) (1 month) group (IIIb2) (0.575 MPa). The difference between different groups was statistically significant as revealed with one way ANOVA test (p<0.05). Tukey's post-hoc test showed non-significant (p>0.05) differences between {(Apple Vinegar+AH Plus) and (NaOCl +AH Plus) (1 week)}, {( EDTA+ AH Plus), (Apple Vinegar+ AH Plus) and(NaOCl+ AH Plus) (1 month)}, {(EDTA+ ZnO nanosealer) and (NaOCl+ ZnO nanosealer) (1 month)}. Conclusions: AH Plus sealer had the highest push-out bond strength when used with EDTA, ZnO nanosealer had the highest push –out when used with apple vinegar and all AH Plus subgroups had higher push-out bond strength than ZnO nanosealers subgroups