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199838

Programmed death protein 1 (PD1) mRNA expression as a screening and diagnostic tool in colon cancer

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Last updated: 22 Jan 2023

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Abstract

Purpose: Colon cancer is one of the most frequent malignant tumors worldwide. Studies showed that in patients with colon cancer, immune system is generally compromised. Correlation of PD1 expression and numerous types of cancer such as colorectal cancer have been well illustrated. The higher levels of PD1 expression correlates with poorer prognosis.
In our study, we aim to investigate the associations between PD1 gene expression and susceptibility to CRC.
Methods: This study was carried out on 50 patients with colon adenocarcinoma, 50 patients with benign colon polyp and 50 apparently healthy persons served as controls. All subjects were exposed to full history taking, general clinical examination. Complete blood count, liver and kidney function, determination of serum tumor markers (CEA and CA19-9). Estimation of PD1 Gene expression by real-time PCR was done.
Results: The mean PD1 gene expression was 5.8% in cancer patients compared to 0.9% in benign polyps group and 0.04 in normal people. The sensitivity and specificity of PD1 expression in our study was 98% and 95% respectively. Higher PD1 gene expression had statistically significant relation with tumor stage (p=0.001) and presence of metastases (p=0.003).
Conclusions: The level of PD1 can be used to differentiate between colon cancers and begin adenomas. PD1 could be used as a prognostic marker in colon cancer.

DOI

10.21608/secioj.2021.199838

Keywords

colorectal cancer, programmed death protein-1, Real- time PCR

Volume

9

Article Issue

4

Related Issue

27629

Issue Date

2021-10-01

Receive Date

2021-09-13

Publish Date

2021-09-14

Page Start

254

Page End

262

Print ISSN

2537-0995

Online ISSN

2314-8500

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https://secioj.journals.ekb.eg/article_199838.html

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https://secioj.journals.ekb.eg/service?article_code=199838

Order

9

Publication Type

Journal

Publication Title

SECI Oncology Journal

Publication Link

https://secioj.journals.ekb.eg/

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Article

Created At

22 Jan 2023