Dermatophytes are classified into 3 genera of fungi that commonly involve keratins tissue types; skin, nails and hairs in mammals. Ultraviolet radiation are used in the treatment of these fungi which cause DNA damage leading to inactivation of the pathogenic fungus. The present study was aimed to evaluate the ultraviolet (UV) radiation influence on the dermatophytes lesion growth that attack human and animal. Thirty isolates of dermatophytes were isolated from animals (skin scraping) and humans (skin scraping, hair and nail). Five species were identified viz., Trichophyton mentagrophyte, Trichophyton interdigitale, Trichophyton tonsurans, Trichophyton violaceum and Microsporum canis. UV-A, was found to be more active in delaying the dermatophytes colony growth in some current fungi widespread when combined with antifungal therapy to treat skin superficial fungal diseases, which caused dermatophytes lesion in human and animal. The present approach that a combination of antifungal drugs and UV-A to treat apparent dermatophytes diseases could reduce the therapeutic period and dosages which improve patients compliance.