The aim of this study was focused on investigating the possible protective effect of Bee Venom (BV) against gamma radiation induced damage and inflammation in male rats. Gamma-irradiation (6 Gy) resulted in a significant elevation in the level of tumor necrotic factor-alpha (TNF-α) , interleukin-6 (IL-6) , creatinine kinase-MB (CK-MB) and cardiac troponin I (cTnI), lactate dehydrogenase (LDH), creatine phosphokinase (CPK), serum aspartate transaminase (AST) and alanine transaminase (ALT) , serum glucose, malondialdehyde (MDA) concentration and xanthine oxidase activity associated with remarkable decrease in insulin level, glutathione content (GSH) and the activity of xanthine dehydrogenase (XDH), superoxide dismutase (SOD) and catalase (CAT) in heart and liver tissues compared to control group. Injection of BV (5ml/kg b. wt. / day/6weeks) to γ-irradiated rats was found to offer protection against γ-irradiation induced oxidative stress and significantly ameliorated the changes occurred in the above investigated biochemical parameters. It could be concluded that Bee venom clarified a modulatory role against gamma radiation induced oxidative damage and inflammation in the heart and liver tissues.