Seeds are known to be a rich house of nutrients despite being small in size. They also contain healthy monounsaturated fats, polyunsaturated fats and many important vitamins, minerals and antioxidants.When consumed as part of a healthy diet, can help reduce blood sugar, liver health,cholesterol and blood pressure. This investigation aimed to study the effect of mixture of chia, flax and milk thistle seeds on carbon tetrachloride (CCl₄) induced liver toxicity in female rats. Twenty female rats weighting (160±5g) were divided into 4 equal groups (n= 5 rats), one group was kept on basal diet as control negative while the other 3 groups were injected by CCl₄ in paraffin oil 50 % v/v (2ml/kg.b.w.t.) twice a week for two weeks to induce liver intoxication; one of them was kept as basal diet and left as control positive (+ve),while the other two groups have the basal diet supplemented with 10 &15 % mixture of chia, flax and milk thistle seeds respectively, Liver function and histology were assessed after 4 weeks. At the end of the experimental period (30 days), blood samples were collected for serum separation to determine serum liver enzymes; Glutamate Oxaloacetate Transaminase (AST), Alanine Amino transferase (ALT) and Alkaline Phosphatase (ALP),total protein, albumin, total cholesterol, triglycerides,lipids profile fractions, antioxidant activities and oestradiol hormone.The obtained results concluded that the mixture of the three seeds at two concentrations 10 and 15% caused a significant improvement in liver enzymes, lipid profile, antioxidant activities and oestradiol hormone (P ≤ 0.05) in the intoxicated female rats by CCl₄. In conclusion, feeding rats basal diet supplemented with mixture of chia, flax and milk thistle seeds at10,15% have improved in the levels of liver enzymes,lipid profile, antioxidant and oestradiol hormone in female rats with intoxified liver by CCl₄.