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117365

Co-therapeutic effects of metformin and Sidr fruits and leaves in experimental diabetic rats

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Last updated: 22 Jan 2023

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Abstract

Many diabetic patients prefer to use herbal medicine and treated with conventional drugs, concurrently. The main objective of this study was to evaluate the co-therapeutic effects of Sidr(Zizyphusspina-christi L.) fruits or leaves and metformin in diabetic rats. Forty two adult male albino rats were used and divided into seven groups (G1-G7). G1 was kept on basal diet (negative control), while other groups were subcutaneously injected with alloxan (120 mg/kg body weight).G2 was kept untreated as a positive control, while the other alloxan -injected groups were treated with metformin (100 mg/kg body weight) as a reference drug (G3), or fed on basal diet supplemented with 5% of either Sidr fruits (SFP) or leaves powder (SLP) (G4 and G5, respectively). G6 and G7 received combined treatments (metformin + SFP and metformin + SLP, respectively). The curative period lasted for 4 weeks. By its end, body weight gain (BWG) and relative weights of liver, kidneys and pancreas were calculated. Fasting glucose, insulin, amylase activity, liver glycogen, lipid profile as well as liver and kidney functions were determined. The typical manifestations of diabetes were noticed in untreated diabetic group including hyperglycemia and hypoinsulinemia.Moreover, a significant increase in serum amylase activity and relative liver and kidney weights was noticed associated with dyslipidemia, liver and kidney dysfunction and a significant decrease in BWG and absolute pancreas weight along with marked histopathological abnormalities in pancreas tissue.SFP was more effective than SLP in normalizing body weight, pancreas weight and liver antioxidant status, while the capability of SLP was higher in correcting the level of serum low density lipoprotein cholesterol (bad cholesterol). In general, receiving combined treatments was more efficient than receiving metformin alone.In conclusion, Sidr fruits and leaves can help metformin in the treatment of diabetes mellitus.

DOI

10.21608/ejnh.2020.117365

Keywords

Diabetes mellitus, alloxan, Zizyphusspina-christi L, Metformin, experimental rats

Authors

First Name

Samah

Last Name

El-Hashash

MiddleName

A.

Affiliation

Nutrition and Food Science Dept., Faculty of Home Economics, Al-Azhar University, Egypt.

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Volume

15

Article Issue

1

Related Issue

17687

Issue Date

2020-01-01

Receive Date

2020-10-06

Publish Date

2020-01-01

Page Start

17

Page End

37

Print ISSN

1687-7950

Online ISSN

2535-1559

Link

https://ejnh.journals.ekb.eg/article_117365.html

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https://ejnh.journals.ekb.eg/service?article_code=117365

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2

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Original Article

Type Code

443

Publication Type

Journal

Publication Title

Egyptian Journal of Nutrition and Health

Publication Link

https://ejnh.journals.ekb.eg/

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Article

Created At

22 Jan 2023