In traditional Chinese medicine, ganoderma lucidum are widely used as medicine for a variety of diseases, such as chronic bronchitis inflammation, hyperlipidemia, hypertension, neurasthenia, hepatitis, leukopenia, and adjuvant treatment of cancer . The aim of this study was to evaluate its effect against cisplatin - induced nephrotoxicity in adult male albino rats . Forty five animals were classified into 5 groups 9 rats for each group . Group (1) fed on basal diet (negative group); groups (2 - 5) were injected with cisplatin ( 5 mg / kg body wt. ) intraperitoneuly to induce nephrotoxicity in rats ; group ( 2 ) fed on the basal diet as positive control group (+Ve) ; remained groups fed on the basal diet and supplemented with ganoderma lucidum at 5 , 10 and 15 g / kg diet , respectively . Results revealed that positive control group had significant increase in serum levels of creatinine , urea , uric acid , urea nitrogen and total protein as well as Malondialdahyde ( MOD ) but had significant decrease in serum levels of Superoxide Dismutase (SOD) , Catalase (CAT) , Glutathione Peroxidase (GPX) and Glutathione (GSH) as compared to the negative control groups. While supplemented with different levels of ganoderma lucidum caused significant decreased in serum levels of creatinine , urea , uric acid , urea nitrogen and total protein as well as ( MOD ) , but had significant increased in serum levels of (SOD) , (CAT) , (GPX) and (GSH) as compared to the positive control group. From the present results it can be concluded that ganoderma lucidum feeding possess a therapeutic effect against cisplatin that induced nephrotoxicity in rats and inhibit oxidative stress. According to above results the consumption of ganoderma lucidum can be used to treat nephrotoxicity