Recently, red radish sparked much interest in health and nutrition. So this study aimed to clarify the effect of red radish roots juice on obese rats. The chemical analysis of the phenolic content and antioxidant activity of the red radish roots juice were determined. An experiment performed on 36 male rats distributed into 6 equal groups. The first group kept as normal control, fed on a basal diet. While other groups fed on a high-fat diet to induce obesity. Obese rats, group (2) left as a positive control. Three obese groups (3, 4 and 5) received red radish roots juice (1, 2 and 3 ml/kg BW/ day) with a high fat-diet. After 6 weeks, biological parameters (feed intake FI, bodyweight gain BWG, body mass index BMI, and Feed efficiency ratio FER) calculated, and biochemical analysis (serum leptin, adiponectin, oxidative stress, tumor necrosis factor-alpha (TNFα), total antioxidant capacity, and lipid profile) estimated. Results confirmed that a high level of ferric reducing antioxidant (FRAP) and total radical-trapping antioxidant parameter (TRAP) in a positive control group. Red radish roots juice affected a significant decrease in leptin, adiponectin, TNF-α, and total oxidant capacity, while increasing the total antioxidant capacity as compared to the positive control group. Also, lipid profile normalization showed in group 5 that received a high-fat diet plus 3 ml red radish roots juice. Therefore, this study concluded that the consumption of red radish as a functional food for a delay may protect the risk of adipose tissue.