This study was performed to assess the hypoglycemic and Antiatherosclerotic effects of dried red radish roots on hypercholesterolemic diabetic rats. Red radish roots contained good quantities of bioactive compounds as phenolic compounds, flavonoids and anthocyanin also its antioxidant activity. Rats were randomly divided into two main groups, the first group is normal control, and the second is the hypercholesterolemic diabetic rats which were divided into four subgroups (8 rats each) as follow: subgroup 1 served as positive control group, subgroup 2, 3 and 4 were fed on the diet containing 2.5%, 5% and 7.5% of dried red radish roots powder for 6 weeks respectively. After completing the treatment period, blood samples were collected for used to the biochemical analysis as blood glucose, insulin and lipid profile. Heart were taken for aortic histopathological. The results indicated that treatments with dried red radish roots significantly decreased the levels of blood and urine glucose, lipid profile, hepatic lipid and atherogenic indices compared to positive control group and improved the level of insulin and histopathological changes in the aorta of hypercholesterolemic diabetic rats. In conclusion, red radish roots as a source of antioxidant compounds had hypoglycemic effect, improved lipid metabolism and prevent cardiovascular diseases.