In the present study, gamma radioactivity in phosphate samples from El-Naser phosphate quarry in El-Mahamid area, Egypt was evaluated using gamma spectrometry with NaI (Tl) detector. The assessment of the radioactivity was carried out by computing several parameters of hazard indices and radiological doses for the samples analyzed in order to assess the radiological risks resulting from phosphate mining activities. The results also were compared with available similar studies and relevant reference and statistically analyzed using Statistical Program for Social Science (SPSS 25.0). The average activity concentrations of 452.06 ± 14.7, 49.34 ± 1.68 and 360.70 ± 12.49 Bq/Kg were obtained for 226Ra, 232Th and 40K respectively. The average activity concentration obtained for 226Ra was twelve times higher than the world average value recommended by UNSCEAR. The average values of all hazard indices and radiological doses discussed in this study were found to be higher than the world average values recommended by UNSCEAR. This study provides information about radiological threat of phosphate activities on health and environment which require an additional radiological attention for protection.