Round up, a worldwide herbicide has cellular and genotoxic effects. Round up's genotoxicity was tested by detecting the expression of the steroidogenic acute regulatory, aromatase, estrogen and androgen receptor genes in testicular cells. Its cytotoxicity was examined by measuring caspase 3 gene expressions. Genes' expressions were assessed by RT- PCR. Testicular lesions were detected by histopathological examination. We determined the role of vitamin E in reducing its toxic effects. Our study determined that Round up toxic doses caused endocrinal disruption through alteration of genes expression and effects on cellular integrity by increasing apoptosis. Administration of the vitamin E during exposure can reduce and protect against these endocrine cytogenotoxic effects.