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41029

POSSIBLE PROTECTIVE EFFECT OF ALPHA LIPOIC ACID AGAINST RICIN INDUCED HEPATOTOXICITY IN ADULT MALE ALBINO RATS

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Last updated: 22 Jan 2023

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Abstract

Introduction: Ricin is one of the most potent and deadly plant toxins known. It induces oxidative stress and was exploited as a poison for bioterrorism due to its characters. Developing an effective antidote for ricin toxicity becomes a reliable way against its potential biothreat. Alpha lipoic acid (ALA) being an ideal antioxidant, was chosen as protection against ricin toxicity. Aim of the work: The current study was carried out to confirm the hepatotoxic effect of ricin using the electron microscope (EM) and to assess the potential protective effect of ALA against ricin hepatotoxicity and lethality. Material and methods: The current study was conducted on 60 adult male albino rats. Rats were divided into four groups. The first group (10 rats) was used as control group. Group II (10 rats) was injected with ALA (100mg/kg intraperitoneal). Group III (20 rats) was injected once with ricin (25ug/kg subcutaneous). Group IV was injected with ALA (100 mg/kg) 15 minute prior to and immediately after ricin injection. Survival time was recorded and liver samples were taken for examination under EM. Results: Injection of ALA together with ricin significantly prolonged the survival time of the rats in group IV more than those in group III. Electron microscopic examination of liver specimens obtained from group III revealed hepatocytes with either euchromatic nuclei, shrunken hyperchromatic nuclei or nuclei with marginated chromatin. Hugely dilated degranulated rough endoplasmic reticulum (RER), many lipid droplets and electron dense mitochondria were detected. Treatment of animals with ALA resulted in less ultra structure damage. The hepatocytes showed either euchromatic nuclei or nuclei with marginated chromatin. Their cytoplasm exhibited slightly dilated RER, electron dense mitochondria and some vacuoles. Conclusion: It was concluded that ricin is a hepatotoxic substance causing ultra structure damage. Furthermore, ALA proved to be beneficial in prolonging the survival time and decreasing the toxic effect of ricin on the liver. Recommendations: Further studies are recommended to detect the underlying mechanism of ricin hepatotoxicity and to find more protective substances against ricin toxicity

DOI

10.21608/ejfsat.2016.41029

Keywords

Ricin, Alpha lipoic acid, Hepatotoxicity, Electron Microscope

Authors

First Name

Amira A.

Last Name

Wahdan

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Affiliation

Departments of Forensic Medicine and Clinical Toxicology , Faculty of Medicine, Tanta University

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Orcid

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First Name

Naglaa

Last Name

Sarhan

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Affiliation

Department of Histology, Faculty of Medicine, Tanta University

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Volume

16

Article Issue

2

Related Issue

973

Issue Date

2016-12-01

Receive Date

2016-09-15

Publish Date

2016-12-01

Page Start

209

Page End

221

Print ISSN

1687-0875

Online ISSN

2535-1915

Link

https://ejfsat.journals.ekb.eg/article_41029.html

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https://ejfsat.journals.ekb.eg/service?article_code=41029

Order

13

Type

Original Article

Type Code

429

Publication Type

Journal

Publication Title

The Egyptian Journal of Forensic Sciences and Applied Toxicology

Publication Link

https://ejfsat.journals.ekb.eg/

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Article

Created At

22 Jan 2023