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45928

Evaluation of mini-FLOTAC method for diagnosing intestinal parasitic infections

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Last updated: 22 Jan 2023

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Abstract

Background:  Intestinal parasitic infections (IPIs) are widely distributed throughout the world and have been identified as one of the most significant causes of illnesses and diseases. Accurate diagnosis is essential for proper management and to guide the design, implementation and monitoring of programs for community control of infectious diseases. Objective: To evaluate and compare mini-FLOTAC technique with the widely used formol-ethyl-acetate concentration method (FECM) in IPIs detection. Material and Methods: One hundred fecal samples were randomly collected from different laboratories. All samples were microscopically examined using mini-FLOTAC and FECM. Iodine and modified Zeil-Neelsen stains were used to confirm the presence of protozoa cysts, and oocysts of Cryptosporidium spp. and C. cayetanensis, respectively. Results: About two thirds of samples (68%) were positive; 70.6% (48/68) contained only one parasitic infection by at least one method. Generally, the most frequent was Cryptosporidium spp. 35.3% (24/68) followed by E. histolytica/dispar 23.5% (16/68) and G. intestinalis 14.7% (10/68). It was observed that mini-FLOTAC detected the highest number of helminthes infections (100% sensitivity) in contrast to 41.1% for protozoa whereas FECM was the most sensitive approach for protozoa infections (98.2% sensitivity) in contrast to 68.7% for helminthes. Conclusion: Mini-FLOTAC is a safe rapid device for microscopic examination of stool samples with high sensitivity, affordability, and appropriateness of diagnosis in resource-limited settings where IPIs are widespread

DOI

10.21608/puj.2019.13212.1045

Keywords

Formol-ethyl-acetate concentration, intestinal parasitic infections, Mini-FLOTAC

Authors

First Name

Nada

Last Name

El-Nadi

MiddleName

-

Affiliation

Medical Parasitology Department, Faculty of Medicine, Sohag University, Egypt

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Orcid

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First Name

Amal

Last Name

Ahmed

MiddleName

-

Affiliation

Medical Parasitology Department, Faculty of Medicine, Sohag University, Egypt

Email

moustafad658@yahoo.com

City

-

Orcid

-

First Name

Noha

Last Name

Ahmed

MiddleName

-

Affiliation

Medical Parasitology Department, Faculty of Medicine, Sohag University, Egypt

Email

nohasammer@yahoo.com

City

-

Orcid

-

First Name

Amany

Last Name

El-Laah

MiddleName

-

Affiliation

Medical Parasitology Department, Faculty of Medicine, Sohag University, Egypt

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-

City

-

Orcid

-

Volume

12

Article Issue

2

Related Issue

7111

Issue Date

2019-08-01

Receive Date

2019-05-29

Publish Date

2019-08-01

Page Start

147

Page End

152

Print ISSN

1687-7942

Online ISSN

2090-2646

Link

https://puj.journals.ekb.eg/article_45928.html

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https://puj.journals.ekb.eg/service?article_code=45928

Order

9

Type

Original Article

Type Code

426

Publication Type

Journal

Publication Title

Parasitologists United Journal

Publication Link

https://puj.journals.ekb.eg/

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Article

Created At

22 Jan 2023