Background: Obesity is a serious medical health problem in the world. Orlistat drug and green tea herb have an important anti-obesity effect. Aim of the work: This study evaluated the chronic toxic effects of orlistat and green tea extract on testes of 36 adult male albino rats after 45 &90 days with orlistat & GTE. Material and methods: The study was conducted for 90 days on 36 male adult albino rats, divided into three groups; (each one subdivided into 2 equal subgroups). Group I: control group, group II: treated orally with orlistat at a dose of 500 mg/kg/day. Group III: treated orally with GTE at a dose of 700 mg/kg/day. At the start of the experiment, after 45 & 90 days body parameters of all rats were calculated before scarification then, their blood and testicular tissues were subjected to biochemichal and semen analysis, while the remaining testicular tissues were stained for histopathological and immune-histochemical studies. The results: administration of orlistat for 45 days showed mild histopathological changes. after 90 days, there were a significant decrease of testosterone with deterioration of sperm motility &shape abnormalities percentage & sperm count without effect on LH, moderately positive cytoplasmic and nuclear caspase 3 reaction & no changes in periodic acid schiff (PAS) stain. GTE administration showed time dependent decreased testosterone, increased serum LH, deteriorated semen parameters, marked histopathological changes & thickening in basement membrane of seminiferous tubules with PAS. Strong positive cytoplasmic and nuclear caspase 3 reaction. Oxidative stress on testes occurred after 90 days only in GTE group. Conclusion: chronic consumption of GTE induced more weight loss and testicular toxicity compared with orlistat. Recommendation: Abuse of orlistat and GTE should be avoided and restricted by medical prescription to avoid their toxicities.