Background: At the crime scene, handprints are one of the greatest valuable evidences. Sometimes, latent impressions of hands may be the only evidence left in the crime scene. Using several anthropometric parameters on crime scene evidences as handprint for suggesting stature and sex can play an important role in decreasing number of suspects, in our study we aim to assess the feasibility of handprint measurements in estimation of stature and sex in the local population of Sharkia Governorate. Method: The analyzed study sample consists of 127participants (62 males and 65 females) right-handed between age of (18 and 60 years ) from people of Sharkia governorate, stature was measured and handprints were scanned by (CanoScan lide120) .Handprint parameters were processed and taken using Image j program measuring 21 handprint measurements. Results: As regarding the stature, showed that there is a marked difference between males and females, where males are higher in length than females in this study and all the taken measures showed noticed differences between them, Estimation of Stature from handprint measurements by equations from regression (simple linear and best fitting multiple linear models), in regard to sex estimation, all 21 handprint measurements are sexually dimorphic. In the stepwise analysis of the handprint variables, two measurements were selected: handprint breadth and the distal phalangeal length of the thumb. Conclusion: Stature and sex can be estimated from handprint measurements.