ABSTRACT
Background: Tramadol is an opioid pain medication used to treat premature ejaculation and as an antidepressant also young population abuse tramadol. Long-term administration of tramadol has negative impacts on many organs like ovary and testis. Objective: The aim of study was to assess effect of tramadol on testes and ovaries of adult albino rats and to elucidate possible protective effect of Aloe Vera gel. Methodology: one hundred adult albino rats were used in present study. Animals were divided into five groups: control group, solvent control group, Aloe Vera gel group, tramadol group and tramadol and Aloe Vera gel group, testes and ovaries were excised and specimens were processed for light, electron microscopic examination, blood samples were obtained to be tested for: testosterone, malondialdehyde MDA, and reduced glutathione GSH. Results: The present work detected a highly significant reduction in serum testosterone, and GSH levels and a highly significant increase in serum MDA levels in tramadol group as compared to control group. A highly significant increase in serum testosterone, and GSH levels and a highly significant reduction in MDA levels in tramadol and Aloe Vera gel group as compared to group tramadol and these results were non-significant as compared to controls. Tramadol-treated male rats showed dilatation in seminiferous tubules and vacuolation, testicular atrophy, and spermatogenic arrest, ovaries of tramadol-treated rats showed atretic follicles, with hyperplasia of corpus luteum (CL) and vacuolation. All histopathological changes, were ameliorated when Aloe Vera was co-administered with tramadol. Conclusion: Tramadol has harmful effect on testes and ovaries of rats. Administration of Aloe Vera accelerates healing of testicular tissue structure and has positive effect on testosterone level.