Applicability of some isolates and Eminent for control sugar beet cercospora leaf spot disease which cused by C.ercospora beticola was inestigated. The fungicide, Eminent was the superior in vitro treatment in suppressing growth of the pathogenic fungus. Bacillus subtilis (B1), B. subtilis (B2) and Trichoderma koningii (T1) were the most antagonistic against the pathogen. Mycelia of Cercospora beticola were in vitro partially prevented due to application of Eminent, by which its IC50 reached 1.20 ppm. Whereas 88.12 % of the pathogenic fungus were inhibited due to Trichoderma koningii (T1). Additionally, Relative power of antibiosis (RPA) reached their maximal of 2.11 by B. subtilis (B1). Under field conditions, disease severity was reduced to 20.0 and 21.0 % by Eminent during both seasons, respectively, followed by B. subtilis (B1) and T. koningii (T1). Efficiencies of the tested Epicoccum nigrum isolates for controlling sugar beet Cercospora leaf spot disease were not as expected. The enzyme activities of Peroxidase (POX), Catalase (CAT), and Polyphenol oxidase (PPO) was increased in sugar beet to control C. beticola, and induce systemic acquired resistance. Accordingly, chlorophyll content, total soluble solids (T.S.S), sucrose % and root productivity per ton fed.-1 were also enhanced due to the use the superior control of Eminent, Trichoderma koningii (T1) and Bacillus subtilis (B1), respectively.