This work was done at Sakha Agricultural Research Station, Kafr El-Sheikh Governorate, Egypt during three seasons (2018–2020) to assess genetic response to selection and some genetic estimates i.e. phenotypic variance, phenotypic and genotypic coefficients of variability (PCV, GCV) and heritability for boll weight, lint%, lint yield, fiber length, fiber fineness and fiber strength in two extra-long staple cotton crosses: Giza96 x Giza76 (pop.1) and Giza93 x Pima S1 (pop.2) at three generations F2-F4. Results showed significant differences among generations for most traits. Phenotypic variance, PCV, GCV and heritability were decreased as generation proceeded for all traits, indicating more homogenous and uniform in advanced generations. Selected families from F2 and F3 generations had higher mean performance than F3 and F4 realized means. The predicted expected estimates of selection in F2 to F3 and F3 to F4 generations were greater than their corresponded estimates in F3 and F4 generations for all traits. Predicted narrow sense heritability values were higher than realized ones and pop.1 values were higher than pop.2 for all generations. The improvements obtained from the second cycle of selection were greater than that obtained from the first cycle for the traits BW, L%, Mic. and Press. in both populations, whereas, the improvements obtained from the first cycle were greater for the traits LY/P and FL in both populations. Recurrent selection improved the studied traits simultaneously in the desired direction, the two populations varied in their response to selection as the second population showed higher responses for most traits.