This experiment was conducted to evaluate that effect feed withdrawal period on growth performance of broiler chickens. 180 chicks at one day of age were divided into three treatments groups with 6 replicates of 10 birds. First group of chicks as control group was fed basal diets (commercial broilers diets 23% crude protein as starter; 21% crude protein as grower and 19% crude protein as finisher, respectively, until 35 days of age). The second groups fed the same control diets but the feed withdrawal for one hour per day at night. The third group fed the same control diets but the feed withdrawal for two hour per day and one hour at afternoon and one hour at night (from 8-35 days of age). While feed consumption was not different among these three groups, both final body weight and body weight gain were higher in chicks feed withdrawal for 2 h/d; however feed conversion ratio was decreased. Feed withdrawal 2h/d decreased abdominal fat compared with control group while, carcass, muscles, liver and gizzard weights were not affected. Furthermore, feed withdrawal decreased plasma total cholestrol, while neither glutamate oxaloacetate transaminase (GOT) nor glucose were not affected. These results suggested that feed withdrawal had positive effects on growth performance and plasma lipid profiles in broilers chickens.