Multilocation and on stations trials were conducted during 2013 and 2014 to evaluate the performance of twelve rice genotypes under three locations; Sakha (Kafer El-Sheikh Governorate), Gemmiza (El-GharbiaGovernorate) and Zarzoura (El-BehiraGovernorate). The experiments wereconducted using Randomized Complete Block Design with four replications. Combined analysis of variance among the different locations was done. The results obtained showed highly significant differences among rice genotypes, locations, and genotypes by locations interaction. The rice genotypes recorded the highest values of grain yield at Gemmiza location, surpassing significantly the other two locations. These results indicate that the studied genotypes responded differently to the different environmental conditions suggesting the importance of evaluation of genotypes under different environments in order to identify the best genetic make up for a particular environment. The rice genotypes GZ9523-2-1-1-1, Giza 179 and Sakha 101 recorded the highest values of grain yield under the three locations, of Sakha, Gemmiza and Zarzoura during 2013 and 2014 seasons compared with the other genotypes, indicating that those genotypes have a good adaptation under different environments conditions. Concerning the grain quality characters among the three locations, all characters were not affected significantly in both seasons, but the differences among genotypes were significant. Stem borer infestation was affect by location and genotype while, rice blast infection differed significantly among rice genotypes. The highest values of infection of rice genotypes to stem borer were observed at Gemmiza location compared with the other two locations. Sakha101 was resistance to stem borer while, Giza177, Sakha105, Giza179, GZ9461-4-2-3-1, GZ9577-4-1-1, GZ9626-2-1-1 and GZ9807-6-3-2-1 were moderate resistance. On the other hand, Sakha106, GZ7576-10-3-2-1 and GZ9523-2-1-1-1 were moderate susceptible. However, Giza178 was susceptible to stem borer. Most of rice genotypes under study were resistant to rice blast, except Sakha 101 cultivar, which was highly susceptible under different locations in the two seasons.