The present investigation was conducted at the Rice Research and Training Center (RRTC),Sakha, Kafrelsheikh, Egypt, during 2014 and 2015 growing seasons to evaluate some root characters of some rice genotypes for drought and well-watered conditions and determination of heterosis of some root characters in rice (Oryza sativa, L.) in relation to drought tolerance. Preliminary analysis of variance showed that genotypes were differently influenced by sowing conditions. Growth rate (g day¯¹) differed significantly among the genotypes studied. The genotype, Nerica4 had much greater growth rate than other genotypes under study. It was clear that drought stress reduced total root length for all five genotypes. Root volume differentially decreased to a range from 24.53to 19.17 under drought condition. Numbers of total roots were significantly different under the two sowing conditions. Under well watered, the number of roots increased as compared with drought condition. In addition, analysis of variance showed that root dry weight was higher at the well watered compared to drought, meantime root to shoot ratio decreased under drought. The highest values were recorded under well watered condition. The magnitude of heterosis manifested over mid parent and better parents are presented. Highly significant estimates of heterosis as a deviation from mid parent and better parent were exhibited in all studied crosses for growth rate (g day¯¹) and root length (cm) under normal and drought conditions crosses for growth rate (g day¯¹) and root length (cm) are desirable for drought tolerance in rice.