Two field trials were conducted during two successive seasons of 2018/2019 and 2019/2020 in the Floriculture Farm of Horticulture Department, Faculty of Agriculture, Benha University, Egypt to study the effects of spraying with some growth substances (kinetin, salicylic acid, calcium thiosulfate and potassium silicate) and chemical fertilization (N.P.K) as well as their combinations on flowering and some chemical constituents of Matthiola incana L. plants to enhance flowering quality for gardens bed ornamentation. The obtained results showed that spraying plants with 100 ppm kinetin in addition to NPK fertilization at the highest level (100 N: 200 P: 200 K kg/fed) resulted in the highest values in both seasons in case of fresh and dry weights of inflorescence and flowering portion, length and diameter of the flowering portion, number, diameter and fresh weight of florets, flowering duration, leaf total chlorophylls and total indoles content. This treatment, on the other hand, recorded the lowest values of leaves total phenols content. Conclusively, it is recommended to spray Matthiola incana L. plants with kinetin at 100 ppm supplemented with NPK chemical fertilization at 100 N: 200 P: 200 K kg/fed to obtain the best flowering quality for export