This study was carried out at a private farm in AL-Radisiya city, Markaz Edfu, Aswan Governorate, Egypt on the banks of Nile River and Experimental Laboratory of Horticulture Department, Faculty of Agriculture, Benha University, EL-Qaloubia Governorate, Egypt during the two successive seasons of 2018/2019 and 2019/2020 on chia (Salvia hispanica L.) plant. A study was initiated through various biostimulant and chemical fertilizer aiming to evaluate effectiveness of arbuscular mycorrhiza fungi (AMF) or seaweed extract and/or humic acid as well as chemical fertilizer (N.P and K) and the combination of them on vegetative growth, seed yield, chemical constituent's, fixed oil productivity and fixed oil composition of chia (Salvia hispanica L.) plant. Plants sprayed by biostimulants gave the significantly highest mean values for all of studied characteristics mentioned above, particularly T₆: the combined of mycorrhiza, humic acid, seaweed and recommended dose of NPK followed by T₅: recommended dose of NPK (350:200:100 kg/feddan) in the first and second seasons. Meanwhile, T₄: seaweed (1 ml/l) ranked the third values in parameters mentioned before in this concern. Whereas, the richest fixed oil percentage was scored by T₆, followed descendingly by T₅ in the 1^st and 2^nd seasons. Chromatography analysis of chia fixed oil revealed the identification of four components. i.e. palmitic acid, oleic acid, linoleic acid and α-linolenic acid. The main component was α-linolenic acid (43.34 to 53.28%). It can be concluded that T₆ was the best for improving growth, seed yield, fixed oil productivity, chemical constituents and fixed oil components of Salvia hispanica L. plant.