A field experiment was conducted during the two successive seasons of 2017/2018 and 2018/2019 in the Nursery and Laboratory of Ornamental Plants, Faculty of Agriculture, Minia University to study the effect of compost (7.5, 10.0 and 12.5 ton/fed) and seven treatments of stimulatory substances i.e. control, seaweeds extract (300 ppm), amino acids (200 ppm), ascorbic acid (100 ppm), salicylic acid (50 ppm), active yeast (5 g/l) and Minia azotein (50 ml/plant), as well as, their interaction on corms and cormels productivity of gladiolus and some chemical composition constituents. The obtained results indicated that the three levels of compost significantly increased corm diameter, number of cormels/plant, fresh weights/plant, as well as, chemical composition (photosynthetic pigments and N, P and K%) as compared to control. The maximum values were obtained by using compost at 12.5 ton/fed. Treated plants with any of six used stimulatory treatments considerably increased corm and cormels productivity and chlorophyll a, b and carotenoids content, as well as, the percentage of N, P and K in the leaves. Minia azotein was the most effective than the other treatments in this concern the interaction treatments were obtained due to fertilizing gladiolus with compost at 12.5 ton/fed and inoculation of the soil with either Minia azotein or with the sprayed plants with active yeast