This study aimed to examine the influence ofAloe vera gel at different concentrations as growth enhancer on Eucalyptus citriodora, Hook micropropagation. The experiments were conducted from April 1^st 2019 until January 15^th, 2020 at Plant Tissue Culture Laboratory of Prof. Dr. Abd El-Fatah H. Belal, Fac. Environ. Agric. Sci., Arish Univ. The obtained results presented that, soaking Eucalyptus citriodora explants on (Clorox)^® solution at 20% (v/v) for 20 minutes recorded the highest survival percentage of explants (87.33%) on establishment stage. Furthermore, addition of BA at 1 mg l^-1 in combination with NAA at 0.1 mg l^-1 recorded the highest shoot number/explant, shoot length (cm) and leaf number/shoot (7.33, 4.66 and 9.50, respectively) on multiplication. Also, the maximum shoot number/explant, shoot length (cm) and leaf number/shoot (9.33, 7.00 and 11.50, respectively) were achieved with addition of Aloe vera gel at 20 m l^-1.  Moreover, Murashige Skoog (MS) medium with IBA addition at 1.0 mg l^-1 recorded the maximum growth of Eucalyptus citriodora during rooting stage. i.e., shoot number/explant, shoot length (cm), leaf number/shoot, rooting percentage, root number/explant and root length (cm) (3.44, 8.13, 12.86, 99, 3.27 and 5.07, respectively).  Finally, rooted shoots were highly successful (above 80%) on soil mixture contains peatmoss and sand at a rate of (1:1, v:v).