The present work was carried out during the two successive seasons of 2015 and 2016 at the Experimental Farm of El-Quassassin Horticultural Research Station, Ismailia Governorate, Egypt, to study the effect of complete NPK fertilization rates (control, 50, 75 and 100% from the recommended dose), bio and organic fertilizers (control, EM₁ applied as the rate of 100 ml/shrub and active dry yeast with the concentration of 4 g/las a rate of 2 liters/shrub) and the interaction treatments between them on the growth, (fruits and seeds yields), fixed oil content and its components in seeds of Jatropha (Jatropha curcas) shrubs.The results showed that, the best vegetative growth parameters (increasing percentage of shrub height, number of branches and canopy width/shrub), number and weight of fruits (kg)/shrub, number and weight of seeds (kg)/shrub, the yield of fruits and seeds (ton)/fed, fixed oil percentage, weight of fixed oil (g)/shrub and yield of fixed oil (kg)/fed were recorded from the interaction treatment of 75% complete NPK fertilization + EM₁. The analysis of fixed oil of Jatropha shrub showed that, the treatment of 50% NPK gave the best palmitic acid percentage and the treatment of 50% NPK + EM₁ recorded maximum percentages of stearic and oleic acids, while the treatment of 100% NPK + EM₁ gave the highest percentage of linoleic acid.