The experimental trial was consummated at the nursery of Ornamental Horticulture Department, Fac. Agric., Cairo University, Giza, Egypt during two successive seasons of 2014 and 2015 to study the effect of growing media (sand, clay and mixture (sand + clay at 1:1 v/v) and bio-fertilization (microbine, green power and potasiomag) with 200 ml/pot on the chemical composition of Moringa oleifera (chlorophyll a, b, a + b, carotenoids, carbohydrates and mineral elements (N, P and k %). The results showed that the highest leaf contents of chlorophyll a, b and carotenoids, carbohydrates and leaf N, P and K % were obtained from the most suitable media (sand + clay at 1:1 v/v) combined with biofertilization (microbine, green power and potasiomag).