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Objective: Assessment of the survival rate of human embryos after vitrification/thaw
process using two different freezing (vitrification) media with two different solvents;
Dimethylsulfoxide (DMSO) based medium and 1,2Propandiol (PROH) based medium.
These two solvents act as cryoprotective additives (CPA) that provide a protective effect
for human embryos vitrification.
Subject & Methods: Day2 or day3 divided human embryos were exposed to DMSO or
PROH based media before plunging into Liquid Nitrogen (LN2) storage. Later on, during replacement cycles, the embryos were thawed and their survival rate was evaluated.
Results: Two hundreds and twelve embryos in vitrification process were into two groups.
Group A (114 embryos) were treated with sequential vitrification medium based on
DMSO and Group B (98 embryos) were treated with PROH based medium. Thawed
embryos were transferred to physiological media designed for cleavage stage embryos
and kept in the CO2 incubator for 2 to 8 hours. After vitrification/thawing procedure, the
survival rates of group A (DMSO) and group B (PROH) were 85 survived embryos of 114
(74.6%) and 52 survived embryos of 98 (52%) respectively (P value<0.05).
Conclusion: DMSO based medium used for vitrification/thaw process of day2 or day
3 divided embryos demonstrated a significant higher embryo survival rate than that of
PROH based vitrification medium.
DOI
10.21608/egyfs.2013.257397
Keywords
Keywords: Embryo cryopreservation, dimethylsulfoxide, DMSO, 1, 2 Propandiol, PROH, Vitrification
Authors
MiddleName
-Affiliation
1 Department of Chemistry,
Faculty of Science, Ain Shams
University, Cairo, Egypt,
2 Obstetrics and Gynecology
Department; Faculty of Medicine;
Suez Canal University
Email
mohmsh20@hotmail.com
City
-Orcid
-Link
https://egyfs.journals.ekb.eg/article_257397.html
Detail API
https://egyfs.journals.ekb.eg/service?article_code=257397
Publication Title
The Egyptian Journal of Fertility of Sterility
Publication Link
https://egyfs.journals.ekb.eg/
MainTitle
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