Objectives: Estimation of serum Kisspeptin-1 (Kiss-1)
and beta human chorionic gonadotropin (βhCG) levels at
time of pregnancy diagnosis (Booking time) and at the 12th
gestational week; S1 and S2 samples, in trial to evaluate
the predictability of these levels for the possibility of early
pregnancy loss (EPL).
Patients & Methods: 283 women gave S2 samples, 76
women (26.9%) developed EPL (EPL group) and 207
women had viable fetus (Control group). Blood samples
were obtained for ELISA estimation of serum Kiss-1 and
βhCG. The study outcome was the predictability of S1 levels of both parameters for the possibility of oncoming pregnancy loss.
Results: S1 sample βhCG levels showed non-significant
differences between both groups, while serum Kiss-1 levels were significantly lower in EPL than in control women.
Serum levels of both parameters in S2 sample were significantly lower in EPL than both control women and levels
estimated in S1 samples of EPL women. Incidence of EPL
was negatively correlated with S1 sample serum levels of
both parameters. Regression analysis defined 6500 IU/L
and 318 pmol/L as cutoff points for βhCG and Kiss-1 to
predict 50% hazard for EPL. These cutoff points defined
158 and 181 true cases, respectively with significant difference in favor of Kiss-1 value. ROC curve analysis assured
the sensitivity of lower S1 levels of both parameters for
prediction of 50% hazard of EPL, but area difference under
ROC was significant in favor of low serum Kiss-1 as a significant sensitive predictor for EPL.
Conclusion: Estimation of serum kiss-1 at time of pregnancy diagnosis inversely correlates with incidence of
EPL. Lower serum Kiss-1 levels had significantly higher
predictability for EPL than βhCG. Lower serum Kiss-1 at
the 12th GW or development of EPL assured the predictability of the result obtained at booking time and spared the
need for sequential estimations.