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195242

HPLC Phenolic Profiling of Alcoholic Extracts of Three <i>Abutilon</i> Species

Article

Last updated: 22 Jan 2023

Subjects

-

Tags

Section A: Natural Products & Metabolomics

Abstract

Objectives: This study was aimed to analyzing the phenolic constituents of the 80% methanol extracts of three Abutilon species (A. pannosum, A. hybridum and A. hirtum) (family Malvaceae). Methods: Defatted 80% methanol extracts of A. pannosum, A. hybridum and A. hirtum aerial parts (leaves and stems) were, each, analyzed using high performance liquid chromatographic (HPLC) technique against external standard calibration at λ 280 and 330 nm. Results: Twelve phenolic acids were tentatively identified from A. pannosum, A. hybridum and A. hirtum representing 9.03, 12.23 and 20.51%, respectively. The major identified phenolic acids peaks were that of protocatechuic acid in A. pannosum (1.75%), ferulic acid in A. hybridum (2.09%) and ellagic acid in A. hirtum (4.0%). On the other hand, 21 flavonoids were tentatively identified from A. pannosum representing (53.78%), in comparison with 20 identifiedfrom A. hybridum (42.12%) and 19 identified from A. hirtum (35.68%). Kaempferol 3-O- [2\\-p < /em>-coumaroyl-β-D-glucopyranoside] was the major identified flavonoid glycoside in A. pannosum and A. hybridum methanolic extracts (13.27 and 5.13%, respectively)while quercitrin was the major flavonoid glycoside identified in A. hirtum (3.21%). The major flavonoid aglycones identified were luteolin (3.68%) in A. pannosum, apigenin (9.46%) in A. hybridum and quercetin (5.85%) in A. hirtum. Conclusion: The present study revealed that the investigated extracts are rich in phenolic contents; A. pannosum has the highest concentration of flavonoids and the least concentration of phenolic acids, where A. hirtum has the highest concentration of phenolic acids, showing promising phenolic content for further study of their isolation, identification as well as evaluation of their biological activity.

DOI

10.21608/aprh.2021.92719.1139

Keywords

Abutilon pannosum, A. hybridum, A. hirtum, phenolic constituents, HPLC

Authors

First Name

Nermine

Last Name

Mohammed

MiddleName

M.

Affiliation

Department of Pharmacognosy, Faculty of Pharmacy, Helwan University, Cairo, 11795, Egypt.

Email

nermine.mohammed@pharm.helwan.edu.eg

City

Helwan

Orcid

-

First Name

Amel

Last Name

Kamal

MiddleName

M.

Affiliation

Department of Pharmacognosy, Faculty of Pharmacy, Helwan University, Cairo, 11795, Egypt.

Email

kh.omran@yahoo.com

City

Cairo EGYPT

Orcid

0000-0002-9803-7404

First Name

Mohamed

Last Name

Abdelhady

MiddleName

I. S.

Affiliation

Department of Pharmacognosy, Faculty of Pharmacy, Helwan University, Cairo, 11795, Egypt.

Email

mohibrahem@yahoo.com

City

cairo

Orcid

-

First Name

Eman

Last Name

Haggag

MiddleName

G.

Affiliation

Department of Pharmacognosy, Faculty of Pharmacy, Helwan University, Cairo, 11795, Egypt.

Email

eman.g.haggag@pharm.helwan.edu.eg

City

Cairo

Orcid

-

Volume

5

Article Issue

4

Related Issue

28395

Issue Date

2021-10-01

Receive Date

2021-08-25

Publish Date

2021-10-01

Page Start

371

Page End

376

Print ISSN

2357-0547

Online ISSN

2357-0539

Link

https://aprh.journals.ekb.eg/article_195242.html

Detail API

https://aprh.journals.ekb.eg/service?article_code=195242

Order

2

Type

Short Communication

Type Code

357

Publication Type

Journal

Publication Title

Journal of Advanced Pharmacy Research

Publication Link

https://aprh.journals.ekb.eg/

MainTitle

-

Details

Type

Article

Created At

22 Jan 2023