Objectives: Unraveling mechanisms whereby Pseudomonas aeruginosa becomes resistant to carbapenems through testing 114 non-duplicate P. aeruginosa clinical isolates for their susceptibility to various classes of antibiotics and scrutinizing the production of metallo-β-lactamases (MBLs) by tested isolates. Methods: Susceptibility testing of P. aeruginosa to different antibiotics was determined by Kirby-Bauer disk diffusion method and MBLs production by tested isolates was studied phenotypically and genotypically and PCR products were confirmed by sequencing Results: All tested clinical isolates showed eminent resistance to the majority of tested antibiotics and 14 isolates were imipenem (IPM)-resistant. Furthermore, IPM-resistant isolates were verified to be MBLs-producers. MBLs-encoding genes bla_SIM and bla_SPM genes were detected by PCR where four isolates were found to harbor bla_SPM gene while only one isolate harbored bla_SIM gene. The correct size of PCR products of bla_SIM and bla_SPM genes were sequenced and sequences were submitted to the GenBank databases and assigned the accession numbers KX452682 and KX452683 for bla_SIM-2 and bla_SPM-1, respectively. Conclusion: Here, we report the emanation of P. aeruginosa clinical isolates harboring bla_SPM-1 and bla_SIM-2 genes. This may reflect the substantial increase in the rate of imipenem resistance due to MBL in P. aeruginosa clinical isolates from Egypt. Early detection and infection-control practices are of the best antimicrobial strategy for combating this organism.