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Investigation of Livistona decipiens Leaf Methanol Extract and Evaluation of Antioxidant, Antimicrobial and Cytotoxic Activities

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Last updated: 03 Jan 2025

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Abstract

Objectives: This study aimed to isolate the polyphenolic constituents from the methanol extract of Livistona decipiens Becc leaves and evaluate the antioxidant, cytotoxic and antimicrobial activities of the total methanol extract and ethyl acetate fraction Methods: The ethyl acetate and n-butanol fractions of Livistona decipiens leaf methanol extract were subjected separately to different chromatographic separation techniques. Structures of the isolated compounds were established by different spectroscopic techniques (1H / 13C NMR). Antioxidant activitywas evaluated by DPPH assay, while evaluation of cytotoxicity was done according to MTT cell viability assay. Antimicrobial activity was done by agar diffusion method. Results: seven compounds were isolated from the ethyl acetate and n-butanol fractions, they were identified for the first time from the plant as apigenin-8-C-β-D-glucopyranoside (Vitexin) (1), Quercetin-6-C-β-D-glucopyranoside (2),apigenin-6,8-di-C-β-D-glucopyranoside (Vicenin II) (3), 6-O-methyl Kaempferol 3-O-glucopyranoside (4), luteolin-3-C-gentiobiosyl (5),luteolin-6-C-β-D-glucopyranoside (Isoorientin) (6) and luteolin-8-C-β-D-glucopyranoside (Orientin) (7). The ethyl acetate fraction has shown moderate activity against Gram positive and Gram negatvie bacteria, also it showed moderate antioxidant activity with IC50 = 23 ± 0.74 µg/ml when compared to ascorbic acid IC50 = 14.2 ± 0.35 µg/ml. Also ethyl acetate extract has shown cytotoxic activity on MCF-7 cells (human breast cancer cell line), HepG-2 (human hepatocellular carcinoma) and HeLa cells (human cervical cancer cell line), whereas, the methanol extract has shown lower activity. Conclusion: Livistona decipienshave potential medicinal value being rich in polyphenolics and beingantioxidant, cytotoxic and antimicrobial drug.

DOI

10.21608/aprh.2018.5173.1063

Keywords

Antimicrobial, antioxidant, Cytotoxicity, Livistona decipiens, Polyphenolic

Authors

First Name

Haitham

Last Name

Ibrahim

MiddleName

-

Affiliation

Department of Pharmacognosy, Faculty of Pharmacy, Helwan University, Cairo, Egypt, 11795

Email

haithamali081@gmail.com

City

cairo, egypt

Orcid

-

First Name

Fathia

Last Name

Elshaarawy

MiddleName

-

Affiliation

Department of Pharmacognosy, Faculty of Pharmacy, Helwan University, Cairo, Egypt, 11795

Email

fathiasayed@yahoo.co.uk

City

cairo

Orcid

-

First Name

Eman

Last Name

Haggag

MiddleName

-

Affiliation

Department of Pharmacognosy, Faculty of Pharmacy, Helwan University, Cairo, Egypt, 11795

Email

eman.g.haggag@pharm.helwan.edu.eg

City

Cairo

Orcid

-

Volume

2

Article Issue

4

Related Issue

3784

Issue Date

2018-10-01

Receive Date

2018-09-18

Publish Date

2018-10-01

Page Start

256

Page End

268

Print ISSN

2357-0547

Online ISSN

2357-0539

Article File

APRH_Volume 2_Issue 4_Pages 256-268.pdf

PDF . 407.6kB

Link

https://aprh.journals.ekb.eg/article_17751.html

Detail API

https://aprh.journals.ekb.eg/service?article_code=17751

Order

4

Type

Research Article

Type Code

318

Publication Type

Journal

Publication Title

Journal of Advanced Pharmacy Research

Publication Link

https://aprh.journals.ekb.eg/

MainTitle

Investigation of Livistona decipiens Leaf Methanol Extract and Evaluation of Antioxidant, Antimicrobial and Cytotoxic Activities

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Type

Article

Created At

22 Jan 2023