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Flavonolignans and Biological Activity of Nannorrhops ritchiana Leaves

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Last updated: 03 Jan 2025

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Abstract

Objectives: This study aimed at isolating the polyphenolic compounds of 70% methanol extract ofNannorrhops ritchiana Griff. leaves and assaying the antioxidant and cytotoxic activities of the extract and main fractions. Methods: The methanol extract of the leaves of N. ritchiana was chromatographically fractionated using a bioactivity-guided approach. The isolated compounds were spectroscopically elucidated by UV, MS, 1H/13C NMR and 2D NMR spectroscopic techniques. The radical scavenging activity of the methanol extract and its fractions was evaluated using DPPH (2,2-Diphenyl-1-picrylhydrazyl) radical scavenging assay and their cytotoxic activity was assayed using SRB (3-(4,5-dimethyl thiazol-2-yl)-2,5-diphenyltetrazolium bromide) test against human hepatocellular liver carcinoma (HepG2), human alveolar adenocarcinoma (A549) and human prostate carcinoma (PC3) cell lines. Results: Eight compounds were isolated and identified for the first time from the leaves of N. ritchiana, comprising five flavonolignans;Indocalatin A (4) which is a rare compound that has been reported only once  in nature, 9″-O-glucopyranosyl Salcolin A (5), 9″-O-glucopyranosyl Salcolin B (6) together with their aglycones, Salcolin A (7) and Salcolin B (8) along with three flavone glycosides; Tricin-7-O-rutinoside (1), Orientin (2) and Isoorientin (3). The methanol extract of N. ritchiana showed antioxidant activity with SC50 39.4 ± 1.06 μg/ml, while fraction IIshowed significant effect with SC50 of 6.1 ± 0.24 μg/ml in comparison with ascorbic acid (SC50 1.8 ± 0.35 μg/ml). The methanol extract and the fraction III showed a promising cytotoxic activity against selected cell lines especially the A549 with IC50 9.5 ± 1.98 μg/ml and HepG2 with IC50 8.15 ± 1.3 μg/ml, respectively. Conclusion: N. ritchiana leaf methanol extract is a new source of biologically active compounds, including flavonolignans.
 

DOI

10.21608/aprh.2017.4721

Keywords

antioxidant, Cytotoxicity, DPPH, Flavonolignan, HPLC, Nannorrhops ritchian Griff, SRB

Authors

First Name

Ahmed

Last Name

Essa

MiddleName

F.

Affiliation

Department of Chemistry of Natural Compounds, National Research Centre, El Bohouth St., Dokki, Giza, 12622, Egypt.

Email

ahmedfathyessa551@gmail.com

City

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Orcid

-

First Name

Tahia

Last Name

Mohamed

MiddleName

-

Affiliation

Department of Chemistry of Natural Compounds, National Research Centre, El Bohouth St., Dokki, Giza, 12622, Egypt.

Email

-

City

-

Orcid

-

First Name

Eman

Last Name

Haggag

MiddleName

-

Affiliation

Department of Pharmacognosy, Faculty of Pharmacy, Helwan University, Ain Helwan, Cairo11795, Egypt

Email

eman.g.haggag@pharm.helwan.edu.eg

City

-

Orcid

-

First Name

Ezz EL-Din

Last Name

Elkhrisy

MiddleName

-

Affiliation

Department of Chemistry of Natural Compounds, National Research Centre, El Bohouth St., Dokki, Giza, 12622, Egypt.

Email

ezzeldin183@gmail.com

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-

Orcid

-

Volume

2

Article Issue

1

Related Issue

824

Issue Date

2018-01-01

Receive Date

2017-10-04

Publish Date

2018-01-01

Page Start

44

Page End

55

Print ISSN

2357-0547

Online ISSN

2357-0539

Article File

APRH_Volume 2_Issue 1_Pages 44-55.pdf

PDF . 930.1kB

Link

https://aprh.journals.ekb.eg/article_4721.html

Detail API

https://aprh.journals.ekb.eg/service?article_code=4721

Order

5

Type

Research Article

Type Code

318

Publication Type

Journal

Publication Title

Journal of Advanced Pharmacy Research

Publication Link

https://aprh.journals.ekb.eg/

MainTitle

Flavonolignans and Biological Activity of Nannorrhops ritchiana Leaves

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Article

Created At

22 Jan 2023